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Journal / Biochemistry / Realtid Observation av DNA Strand Exchange…
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Biochemistry

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JoVE Journal Biochemistry
Realtid Observation av DNA Strand Exchange reaktionen medieras av Rad51
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Realtid Observation av DNA Strand Exchange reaktionen medieras av Rad51

Article DOI: 10.3791/59073-v • 06:24 min • February 13th, 2019
February 13th, 2019 •
1, 1, 1, 1
1Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology

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Fluorescens resonans energi överföring-baserade realtid observation system av DNA strand exchange reaktion medierad av Rad51 utvecklades. Med hjälp av protokollen som presenteras här, kan vi upptäcka bildandet av reaktion intermediärer och deras omvandling till produkter, samtidigt också analysera enzymatisk kineticsen av reaktionen.

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Biokemi fråga 144 homolog rekombination DNA strand exchange reaktion Rad51 Swi5-Sfr1 realtid observation fluorescens resonans energiöverföringen (bandet)
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