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Journal / Genetics / Genom-redigering i pattedyr cellelinjer ved hjælp …
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Genom-redigering i pattedyr cellelinjer ved hjælp af CRISPR-Cas
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Genom-redigering i pattedyr cellelinjer ved hjælp af CRISPR-Cas

Article DOI: 10.3791/59086-v • 07:56 min • April 11th, 2019
April 11th, 2019 •
1, 1, 1,2, 1,2
1Genome Institute of Singapore, Agency for Science Technology and Research, 2School of Chemical and Biomedical Engineering, Nanyang Technological University

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CRISPR-Cas er en kraftfuld teknologi til ingeniør komplekse genomer af planter og dyr. Vi detalje her, en protokol til effektivt redigere det menneskelige genom ved hjælp af forskellige Cas endonucleases. Vi fremhæve vigtige overvejelser og design parametre til at optimere redigering effektivitet.

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Genetik spørgsmålet 146 CRISPR Cas9 Cas12a genom-redigering ikke-homologe ende sammenføjning homologi-instrueret reparation
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