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JoVE Journal Chemistry
Forbedre høj viskositet ekstrudering af Microcrystals for tidsopløst seriel femtosekund krystallografi på X-ray lasere
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Forbedre høj viskositet ekstrudering af Microcrystals for tidsopløst seriel femtosekund krystallografi på X-ray lasere

Article DOI: 10.3791/59087-v • 07:26 min • February 28th, 2019
February 28th, 2019 •
1, 1, 1, 1, 1,2, 3,4, 3,4, 1,5, 1
1Division of Biology and Chemistry - Laboratory for Biomolecular Research, Paul Scherrer Institut, 2Photon Science Division - SwissFEL, Paul Scherrer Institut, 3RIKEN SPring-8 Center, 4Department of Cell Biology, Graduate School of Medicine, Kyoto University, 5Department of Biology, ETH Zürich

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Succesen med en tidsopløst seriel femtosekund krystallografi eksperiment er afhængig af effektiv prøven levering. Her beskriver vi protokoller for at optimere ekstrudering af bakterierhodopsin microcrystals fra en høj viskositet mikro-ekstrudering injektor. Metoden bygger på prøve homogenisering med en ny tre-vejs kobling og visualisering med en høj hastighed kamera.

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Kemi spørgsmålet 144 tidsopløst seriel krystallografi lipidic cubic fase bakterierhodopsin Membranproteiner høj viskositet injektor tre-vejs kobling X-ray gratis eletron laser
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