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Journal / Immunology and Infection / Effekten av Anti-c-fms-antikropp på Osteoclast…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Effekten av Anti-c-fms-antikropp på Osteoclast bildande och spridning av Osteoclast föregångare In Vitro
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Effekten av Anti-c-fms-antikropp på Osteoclast bildande och spridning av Osteoclast föregångare In Vitro

Article DOI: 10.3791/59089-v • 07:51 min • March 18th, 2019
March 18th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

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Detta protokoll innehåller dissekering av murina långa ben och benmärg isolering, att generera benmärgen makrofager och producerar Osteoklasterna använder makrofag kolonistimulerande faktor (M-CSF) och receptor activator av nuclear factor Kappa-B-ligand (RANKL) eller tumörnekrosfaktor alfa (TNF-α) och deras gripande av anti-c-fms-antikropp, M-CSF-receptorn.

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Immunologi och infektion fråga 145 osteoclast resorption benmärgen makrofag M-CSF RANKL TNF-α anti-c-fms-antikropp
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