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Journal / Genetics / Manipulation of Gene Function in Mexican Cavefish
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JoVE Journal Genetics
Manipulation of Gene Function in Mexican Cavefish
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Manipulation of Gene Function in Mexican Cavefish

Article DOI: 10.3791/59093-v • 07:01 min • April 22nd, 2019
April 22nd, 2019 •
1,2, 1,2, 1,2, 2,3, 1,2, 2,3
1Department of Biological Sciences, Florida Atlantic University, 2Jupiter Life Science Initiative, Florida Atlantic University, 3Harriet L. Wilkes Honors College, Florida Atlantic University

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Summary
Automatic Translation

We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

Tags

Gene Function Manipulation Mexican Cavefish Genetic Tools Surface Fish Cave Fish Phenotypes Astyanax Mexicanus Postdoc Egg Injection Mold Agarose Needle Puller Glass Pipette Single Cell Eggs Morpholino Injection
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