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Journal / Genetics / Manipulation af genfunktioner i mexicansk Cavefish
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JoVE Journal Genetics
Manipulation af genfunktioner i mexicansk Cavefish
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Manipulation af genfunktioner i mexicansk Cavefish

Article DOI: 10.3791/59093-v • 07:01 min • April 22nd, 2019
April 22nd, 2019 •
1,2, 1,2, 1,2, 2,3, 1,2, 2,3
1Department of Biological Sciences, Florida Atlantic University, 2Jupiter Life Science Initiative, Florida Atlantic University, 3Harriet L. Wilkes Honors College, Florida Atlantic University

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Vi beskriver tilgange til manipulering af gener i den evolutionære modelsystem Astyanax mexicanus. Tre forskellige teknikker er beskrevet: Tol2-medieret transgenese, målrettede manipulation af genomet ved hjælp af CRISPR/Cas9 og knockdown ytringsfriheden ved hjælp af morpholinos. Disse værktøjer bør lette den direkte undersøgelse af gener, der ligger til grund for variationen mellem overflade - og hulen-boligen former.

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Genetik spørgsmålet 146 Life sciences Life Sciences (generelt) cavefish CRISPR gen-redigering transgenese morpholino knockdown
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