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Journal / Genetics / Meksika Cavefish işlevinde Gene manipülasyon
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JoVE Journal Genetics
Meksika Cavefish işlevinde Gene manipülasyon
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Meksika Cavefish işlevinde Gene manipülasyon

Article DOI: 10.3791/59093-v • 07:01 min • April 22nd, 2019
April 22nd, 2019 •
1,2, 1,2, 1,2, 2,3, 1,2, 2,3
1Department of Biological Sciences, Florida Atlantic University, 2Jupiter Life Science Initiative, Florida Atlantic University, 3Harriet L. Wilkes Honors College, Florida Atlantic University

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Evrimsel modeli sistem Astyanax mexicanusgenler manipülasyon yaklaşımları açıklar. Üç farklı teknikler açıklanmıştır: Tol2-aracılı transgenesis, CRISPR/Cas9 ve nakavt morpholinos kullanarak ifade kullanarak genom hedeflenen manipülasyon. Bu araçları doğrudan soruşturma yüzey ve mağaralarda yaşayan biçimler arasında varyasyon yatan genlerin kolaylaştıracaktır.

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Genetik sayı: 146 yaşam bilimleri Yaşam Bilimleri (genel) cavefish CRISPR gen düzenleme transgenesis morpholino devirme
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