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Journal / Genetics / Charakterisierung von Histon Post-translationale…
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Genetics

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JoVE Journal Genetics
Charakterisierung von Histon Post-translationale Modifikation Veränderungen in Hefe Neurodegenerative Proteinopathy Modelle
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Charakterisierung von Histon Post-translationale Modifikation Veränderungen in Hefe Neurodegenerative Proteinopathy Modelle

Article DOI: 10.3791/59104-v • 08:33 min • March 24th, 2019
March 24th, 2019 •
1,2, 1,3, 1, 1, 1, 1, 1,4
1Chemistry Department, Brooklyn College, 2Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York

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Dieses Protokoll beschreibt experimentelle Verfahren zur Charakterisierung von genomweiten Änderungen in der Höhe von Histon post-translationalen Modifikationen (PTM) in Verbindung mit der Überexpression der Proteine zugeordnet ALS und Parkinson in auftretenden Saccharomyces Cerevisiae Modelle. Nach der Trennung der SDS-PAGE werden einzelne Histon PTM Ebenen mit Modifikation-spezifische Antikörper über Western blotting erkannt.

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Frage 145 Neurodegeneration Amyotrophe Lateralsklerose Parkinson Erkrankung Genetik α-Synuclein verschmolzen in TAR DNA-bindendes Protein 43 Sarkom Epigenetik Histon post-translationalen Modifikationen
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