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Journal / Genetics / Kendetegner Histon posttranslationel modifikation …
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Genetics

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JoVE Journal Genetics
Kendetegner Histon posttranslationel modifikation ændringer i gær Neurodegenerative Proteinopathy modeller
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Kendetegner Histon posttranslationel modifikation ændringer i gær Neurodegenerative Proteinopathy modeller

Article DOI: 10.3791/59104-v • 08:33 min • March 24th, 2019
March 24th, 2019 •
1,2, 1,3, 1, 1, 1, 1, 1,4
1Chemistry Department, Brooklyn College, 2Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York

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Denne protokol beskriver eksperimentelle procedurer for at karakterisere genome-wide ændringer i niveauet af Histon posttranslationelle modifikationer (PTM) forekommer i forbindelse med overekspression af proteiner er forbundet med ALS og Parkinsons sygdom i Saccharomyces cerevisiae modeller. Efter SDS-PAGE adskillelse registreres individuelle Histon PTM niveauer med modifikation-specifikke antistoffer via Western blotting.

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Genetik sag 145 neurodegeneration amyotrofisk lateral sklerose Parkinsons sygdom α-synuclein smeltet i sarkom TAR DNA-bindende protein 43 Histon posttranslationelle modifikationer Epigenetik
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