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Journal / Immunology and Infection / Induktion og scoring af graft-versus-Host-sygdom…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Induktion og scoring af graft-versus-Host-sygdom i en xenogen Murinmodel og kvantificering af humane T-celler i muse væv ved hjælp af digital PCR
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Induktion og scoring af graft-versus-Host-sygdom i en xenogen Murinmodel og kvantificering af humane T-celler i muse væv ved hjælp af digital PCR

Article DOI: 10.3791/59107-v • 06:06 min • May 23rd, 2019
May 23rd, 2019 •
1, 1
1Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center

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Her præsenterer vi en protokol til at inducere og score sygdom i en xenogene graft-versus-Host disease (xenogvhd) model. xenoGVHD giver en in vivo-model til at studere immunsuppression af humane T-celler. Derudover beskriver vi, hvordan man opdager humane T-celler i væv med digital PCR som et redskab til at kvantificere immunsuppression.

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Immunologi og infektion human immunsuppression T Cell graft-versus-Host sygdom xenogene gvhd digital PCR CD3
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