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Biochemistry

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JoVE Journal Biochemistry
Fluorescerende visualisatie van mango-Tagged RNA in Polyacrylamide gels via een Poststaining methode
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Fluorescerende visualisatie van mango-Tagged RNA in Polyacrylamide gels via een Poststaining methode

Article DOI: 10.3791/59112-v • 06:06 min • June 21st, 2019
June 21st, 2019 •
*1, *1, 1
1Department of Molecular Biology and Biochemistry, Simon Fraser University
* These authors contributed equally

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Hier presenteren we een gevoelige, snelle en discriminerende post-gel kleuring methode om image-ASE Tagged met RNA mango aptameren I, II, III, of IV, met behulp van een native of denaturerende Polyacrylamide gel elektroforese (pagina) gels. Na het runnen van standaard pagina gels, mango-gecodeerde RNA kan gemakkelijk worden gekleurd met TOT1-Biotine en vervolgens geanalyseerd met behulp van de meest beschikbare fluorescentie lezers.

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Biochemie RNA mango fluorescentie denaturering gels native gels gel kleuring detectiemethode pagina
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