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JoVE Journal Developmental Biology
Vævs præparation og immun farvning af mus craniofacial væv og Undecalcificeret knogle
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Vævs præparation og immun farvning af mus craniofacial væv og Undecalcificeret knogle

Article DOI: 10.3791/59113-v • 10:03 min • May 10th, 2019
May 10th, 2019 •
*1,2, *2, 2
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 2Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan
* These authors contributed equally

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Her præsenterer vi en detaljeret protokol til påvisning og kvantificering af protein niveauer under craniofacial morfogenesis/patogenesen ved immun farvning ved hjælp af mus craniofacial væv som eksempler. Derudover beskriver vi en metode til klargøring og kryoskæring af undecalcificeret hårdt væv fra unge mus til immun farvning.

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Udviklingsmæssige biologi udviklingsmæssige biologi Transgene mus BMP signalering immun farvning craniofacial morfogenesis undecalcified hårde væv kvantificering
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