Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / ATAC-seq test ADN Mitochondrial faible…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
ATAC-seq test ADN Mitochondrial faible contamination de primaires humaines CD4 + Lymphocytes T
Author Spotlight: RNAi Inheritance and ChIP in C. elegans
Journal (Genetics)
Author Spotlight: RNAi Inheritance and ChIP in C. elegans
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
Journal (Genetics)
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
 
Click here for the English version

ATAC-seq test ADN Mitochondrial faible contamination de primaires humaines CD4 + Lymphocytes T

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Nous présentons ici un protocole pour effectuer un test pour la transposase accessible chromatine séquençage (ATAC-seq) sur activé CD4 + lymphocytes humains. Le protocole a été modifié pour réduire au minimum la contamination mitochondrial DNA lectures de 50 % à 3 % grâce à l’introduction d’une nouvelle mémoire tampon de lyse.

Tags

Génétique numéro 145 épigénomique ATAC-seq Tn5 les lymphocytes CD4 + noyaux contamination de l’ADN mitochondriale
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter