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JoVE Journal Genetics
ATAC-seq analisi con DNA mitocondriale bassa contaminazione da primaria umana CD4 + linfociti T
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ATAC-seq analisi con DNA mitocondriale bassa contaminazione da primaria umana CD4 + linfociti T

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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Qui, presentiamo un protocollo per eseguire un test per transposase-accessibile della cromatina (ATAC-seq) la sequenziazione su linfociti CD4 + umani. Il protocollo è stato modificato per ridurre al minimo la contaminazione letture di DNA mitocondriale dal 50% al 3% attraverso l'introduzione di un nuovo buffer di lisi.

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Genetica problema 145 epigenomica ATAC-seq Tn5 linfociti CD4 + nuclei contaminazione da DNA mitocondriale
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