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Journal / Genetics / ATAÇ-seq tahlil birincil insan CD4 + T…
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ATAÇ-seq tahlil birincil insan CD4 + T lenfositleri kirlenme düşük mitokondrial DNA ile
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ATAÇ-seq tahlil birincil insan CD4 + T lenfositleri kirlenme düşük mitokondrial DNA ile

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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Burada, bir tahlil transposase erişilebilir Kromatin (ataç-seq) aktif CD4 + insan lenfositler üzerinde sıralama için gerçekleştirmek için bir iletişim kuralı mevcut. Protokol % 50 mitokondrial DNA okuma yeni bir lizis arabellek başlanarak % 3 bulaşıcı en aza indirmek için değiştirildi.

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Genetik sayı: 145 Epigenomics ATAÇ-seq Tn5 CD4 + lenfositlerinin çekirdek mitokondriyal DNA kirlenme
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