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JoVE Journal Genetics
ATAC-seq analysen med lav Mitokondrielt DNA forurensning fra primære menneskelige CD4 + T-lymfocytter
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ATAC-seq analysen med lav Mitokondrielt DNA forurensning fra primære menneskelige CD4 + T-lymfocytter

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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Her presenterer vi en protokoll for å utføre en analyse for transposase tilgjengelig chromatin sekvensering (ATAC-seq) på aktivert CD4 + menneskelige lymfocytter. Protokollen er endret for å minimere forurensende Mitokondrielt DNA leser fra 50% til 3% gjennom innføring av en ny lyseringsbuffer.

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Genetikk problemet 145 Epigenomics ATAC-seq Tn5 CD4 + lymfocytter kjerner Mitokondrielt DNA forurensning
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