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Journal / Bioengineering / 글로벌 세포 매트릭스 Metalloproteinase 및 3D Hydrogels 신진…
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Bioengineering

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JoVE Journal Bioengineering
글로벌 세포 매트릭스 Metalloproteinase 및 3D Hydrogels 신진 대사 활동 측정
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글로벌 세포 매트릭스 Metalloproteinase 및 3D Hydrogels 신진 대사 활동 측정

Article DOI: 10.3791/59123-v • 07:39 min • January 22nd, 2019
January 22nd, 2019 •
1,2,3, 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

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여기, 프로토콜 캡슐화 및 poly(ethylene glycol) (PEG) hydrogels fluorogenic 매트릭스 metalloproteinase (MMP)와 기능성된에 세포 배양에 대 한 제시는-분해 펩 티 드. 세포질 MMP와 대사 활동 표준 microplate 리더를 사용 하 여 하이드로 겔 문화에서 직접 측정 됩니다.

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생명 공학 문제점 143 Hydrogels 3D 세포 캡슐화 매트릭스 Metalloproteinases 대사 활동 흑색 형광
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