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JoVE Journal Bioengineering
Måling af Global cellulære Matrix Metalloproteinase og metaboliske aktivitet i 3D Hydrogels
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Måling af Global cellulære Matrix Metalloproteinase og metaboliske aktivitet i 3D Hydrogels

Article DOI: 10.3791/59123-v • 07:39 min • January 22nd, 2019
January 22nd, 2019 •
1,2,3, 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

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Her, en protokol, der er præsenteret for indkapsling og dyrkning af celler i poly(ethylene glycol) (PEG) hydrogels functionalized med en fluorogenic matrix metalloproteinase (MMP)-nedbrydeligt peptid. Cellulære MMP og metaboliske aktivitet måles direkte fra hydrogel kulturer ved hjælp af en standard mikrotiterplade læser.

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Bioteknologi spørgsmålet 143 Hydrogels 3D celle indkapsling Matrix Metalloproteinases metaboliske aktivitet melanom fluorescens
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