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Journal / Bioengineering / Medición de metaloproteinasa de la matriz celular …
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JoVE Journal Bioengineering
Medición de metaloproteinasa de la matriz celular Global y actividad metabólica en hidrogeles 3D
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Medición de metaloproteinasa de la matriz celular Global y actividad metabólica en hidrogeles 3D

Article DOI: 10.3791/59123-v • 07:39 min • January 22nd, 2019
January 22nd, 2019 •
1,2,3, 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

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Aquí, se presenta un protocolo para encapsular y cultivo de células en hidrogeles de poly(ethylene glycol) (PEG), funcionalizados con un fluorógenos matriz metaloproteinasa (MMP)-péptido degradable. MMP celular y actividad metabólica se miden directamente las culturas de hidrogel utilizando un lector de microplacas estándar.

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Bioingeniería número 143 hidrogeles 3D encapsulado metaloproteinasas de la matriz actividad metabólica Melanoma fluorescencia de la célula
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