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JoVE Journal Immunology and Infection
Isolatie en adoptief overdracht van hoge zout behandeld antigeen-presenteren dendritische cellen
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Isolatie en adoptief overdracht van hoge zout behandeld antigeen-presenteren dendritische cellen

Article DOI: 10.3791/59124-v • 09:29 min • March 5th, 2019
March 5th, 2019 •
1, 1, 1, 1, 1
1Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center

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Hier presenteren we een protocol om te isoleren van dendritische cellen uit lymfkliertest milt door magnetische cel Sorteren en latere adoptief overdracht naïef muizen. Het model van hoge-zout geactiveerde dendritische cellen werd gekozen om de stapsgewijze procedures voor de overdracht van de geadopteerde en stroom cytometry uitleggen.

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Immunologie en infecties probleem 145 ontsteking dendritische cel isolatie adoptief overdracht dendritische cellen hypertensie immuun cel sorteren Stroom Cytometry
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