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Journal / Immunology and Infection / Brug af enkelt kæde MHC teknologi at undersøge…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
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Brug af enkelt kæde MHC teknologi at undersøge Co-agonisme i menneskelige CD8 + T-celle aktivering

Article DOI: 10.3791/59126-v • 12:09 min • February 28th, 2019
February 28th, 2019 •
1, 1, 1, 1,2, 3, 4, 3, 1,2,5, 6, 1, 1,2,5
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, 4Singapore Immunology Network, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, 6Department of Immunology, Wright-Fleming Institute, Imperial College London

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Denne protokol beskriver brugen af enkelt kæde MHC klasse I komplekser til at undersøge molekylære interaktioner i menneskelige CD8 + T celle aktivering: generation af manipuleret antigen præsentere celler, der udtrykker enkelt kæde konstruktioner, kultur menneskelige CD8 + T celle klon og T-celle aktivering eksperimenter.

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Immunologi og infektion spørgsmålet 144 enkelt kæde MHC human T-celle aktivering T-celle antigen anerkendelse self peptid stabil cellelinie manipuleret antigen præsentere celler PBMC isolation human T-celle degranulering assay human T-celle cytokin produktion
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