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Journal / Immunology and Infection / Bruk enkelt kjede MHC teknologi å undersøke…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Bruk enkelt kjede MHC teknologi å undersøke Co-agonism i menneskelig CD8 + T celle aktivering
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Bruk enkelt kjede MHC teknologi å undersøke Co-agonism i menneskelig CD8 + T celle aktivering

Article DOI: 10.3791/59126-v • 12:09 min • February 28th, 2019
February 28th, 2019 •
1, 1, 1, 1,2, 3, 4, 3, 1,2,5, 6, 1, 1,2,5
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, 4Singapore Immunology Network, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, 6Department of Immunology, Wright-Fleming Institute, Imperial College London

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Denne protokollen beskriver bruken av enkelt MHC klasse I komplekser undersøke molekylære interaksjoner i human CD8 + T celle aktivering: generasjon utviklet antigen presentere celler uttrykke enkelt kjede konstruerer, kultur for menneskelig CD8 + T celle klone og T celle aktivering eksperimenter.

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Immunologi og infeksjon problemet 144 enkelt kjede MHC menneskelige T celle aktivering T celle antigen anerkjennelse selv peptid stabil cellen linje utviklet antigen presentere celler PBMC isolasjon human T celle omfatter degranulering analysen human T celle cytokiner produksjonen
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