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Journal / Biology / 细胞调理介质细胞外囊泡的流式细胞学分析…
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细胞调理介质细胞外囊泡的流式细胞学分析
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细胞调理介质细胞外囊泡的流式细胞学分析

Article DOI: 10.3791/59128-v • 08:32 min • February 12th, 2019
February 12th, 2019 •
*1, *1, 1,2,3, 1
1Cellular and Molecular Cardiology Laboratory, Cardiocentro Ticino Foundation, Switzerland, 2Molecular Cardiology Institute, Dept. of Cardiology, University of Zürich, 3Faculty of Biomedical Science, Università Svizzera Italiana, Switzerland
* These authors contributed equally

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该协议描述了一种可重复的方法, 该方法设计用于细胞培养上清液, 以检测小细胞外囊泡 (ev) 上的表面表位。它利用特定的 ev 免疫沉淀使用珠子与抗体结合, 识别表面抗原 cd9, cd63 和 cd81。该方法对下游流式细胞仪分析进行了优化。

Tags

生物学 第144期 外质体 微囊泡 流式细胞术 外显子表型珠 调理培养基 细胞外泡
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