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Flow Cytometric analyse af ekstracellulære vesikler fra celle-aircondition medier
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Flow Cytometric analyse af ekstracellulære vesikler fra celle-aircondition medier

Article DOI: 10.3791/59128-v • 08:32 min • February 12th, 2019
February 12th, 2019 •
*1, *1, 1,2,3, 1
1Cellular and Molecular Cardiology Laboratory, Cardiocentro Ticino Foundation, Switzerland, 2Molecular Cardiology Institute, Dept. of Cardiology, University of Zürich, 3Faculty of Biomedical Science, Università Svizzera Italiana, Switzerland
* These authors contributed equally

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Protokollen beskriver en reproducerbar metode udviklet til brug med celle kultur analysere at opdage overflade epitoper på små ekstracellulære vesikler (EV). Det udnytter specifikke EV immunoprecipitation ved hjælp af perler kombineret med antistoffer, der genkender overflade antigen CD9, CD63 og CD81. Metoden er optimeret til downstream flow flowcytometri analyse.

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Biologi spørgsmålet 144 exosomes microvesicles flowcytometri exosomes fænotyper perler konditioneret medium ekstracellulære vesikler
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