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Kvantitativ analyse af cellulære sammensætning i avancerede aterosklerotiske læsioner af glat muskel celle afstamning sporingen mus
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Kvantitativ analyse af cellulære sammensætning i avancerede aterosklerotiske læsioner af glat muskel celle afstamning sporingen mus

Article DOI: 10.3791/59139-v • 09:06 min • February 20th, 2019
February 20th, 2019 •
1, 1, 2,3, 1,4
1Heart, Lung, Blood and Vascular Medicine Institute, University of Pittsburgh, 2Robert M. Berne Cardiovascular Research Center, University of Virginia, 3Department of Biochemistry and Molecular Genetics, University of Virginia, 4Division of Cardiology, University of Pittsburgh School of Medicine

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Vi foreslår en standardiseret protokol for at karakterisere den cellulære sammensætning af sene murine aterosklerotiske læsioner herunder systematiske metoder til animalsk dissektion, væv indlejring, skæring, farvning og analyse af brachiocephalic arterier fra atheroprone glat muskel celle afstamning sporingen mus.

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Medicin spørgsmål 144 åreforkalkning aterosklerotisk læsion brachiocephalic arterie væv forberedelse og skæring immunhistokemi immunofluorescens konfokalmikroskopi
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