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Journal / Immunology and Infection / 一种评估和定量中性粒细胞外细胞陷阱形成的高通量检测…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
一种评估和定量中性粒细胞外细胞陷阱形成的高通量检测
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Journal (Immunology and Infection)
Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
 
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一种评估和定量中性粒细胞外细胞陷阱形成的高通量检测

Article DOI: 10.3791/59150-v • 09:59 min • January 29th, 2019
January 29th, 2019 •
*1, *1, 1, 1, 1, 1, 1
1Department of Nephrology, Leiden University Medical Center
* These authors contributed equally

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该协议描述了一种高度敏感和高吞吐量的中性粒细胞细胞外陷阱 (net) 检测方法, 用于通过免疫荧光三维共聚焦显微镜对体外网络形成进行半自动定量。该方案可用于评估 net 在不同刺激后的形成和降解, 并可用于研究潜在的 net 靶向疗法。

Tags

免疫学与感染 第143期 中性粒细胞外陷阱 (net)、中性粒细胞、免疫荧光显微镜、免疫学、全身自身免疫性疾病、anca 相关血管炎、系统性红斑狼疮、狼疮肾炎
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