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JoVE Journal Environment
Frittliggende Leaf analyser for å forenkle Gene Expression studier i potet under infestation av tygge insekt Manduca Sexta
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Frittliggende Leaf analyser for å forenkle Gene Expression studier i potet under infestation av tygge insekt Manduca Sexta

Article DOI: 10.3791/59153-v • 05:56 min • May 15th, 2019
May 15th, 2019 •
1, 2, 2, 1
1Invasive Insect Biocontrol and Behavior Lab, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), 2Genetic Improvement for Fruits and Vegetables Lab, USDA-ARS

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Den presenterte metoden skaper naturlig herbivore skadet plante vev gjennom anvendelse av Manduca Sexta larver til frittliggende blader av potet. Anlegget vev er analyseres for uttrykk for seks transkripsjon faktor homologs involvert i tidlig svar på insekt herbivory.

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Environmental Sciences infestation Manduca Sexta Solanum tuberosum var. KENNEBEC herbivory C2H2 sink finger transkripsjon faktor forsvar Pathway jasmonic syre
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