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Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)
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Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)

Article DOI: 10.3791/59161-v • 08:09 min • August 6th, 2019
August 6th, 2019 •
1, 2, 3, 3
1Mammalian Genetics Laboratory, National Institute of Genetics, 2Misaki Marine Biological Station, The University of Tokyo, 3Shimoda Marine Research Center, University of Tsukuba

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Summary
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Here, protocols for performing microfocus X-ray computed tomography (microCT) imaging of three marine invertebrate animals are explained in detail. This study describes steps such as sample fixation, staining, mounting, scanning, image reconstruction, and data analyses. Suggestions on how the protocol can be adjusted for different samples are also provided.

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Microfocus X-ray CT MicroCT Actinia Equina Harmothoe Sp. Xenoturbella Japonica Marine Invertebrates Morphology Sample Mounting Common Materials Water Clay Tubes Akiteru Maeno National Institute Of Genetics Japan Agarose Distilled Water Microwave Staining Solution 60-millimeter Dish 50-milliliter Tube Forceps Specimen Positioning
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