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Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
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Neuroscience
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JoVE Journal Neuroscience
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

DOI:
10.3791/59163-v

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09:41 min

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March 20, 2019

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, , , ,
1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

Chapters

  • 00:04Title
  • 00:36Cerebellum Dissection and Sections Preparation
  • 03:58Slices Culture and Demyelination
  • 04:55Immunohistochemistry
  • 08:15Results: Myelin Immunostaining in Organotypic Cerebellar Slices
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a method to prepare organotypic slice cultures from mouse cerebellum and myelin sheath staining by immunohistochemistry suitable for investigating mechanisms of myelination and remyelination in the central nervous system.

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Keywords: Myelinating Organotypic Cerebellar Slice CulturesMyelinationRemyelinationCerebellumDissectionImmunostaining

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