Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / A High-throughput Shigella-specific Bactericidal…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
A High-throughput Shigella-specific Bactericidal Assay
Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls
Journal (Immunology and Infection)
Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
Journal (Immunology and Infection)
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
 

A High-throughput Shigella-specific Bactericidal Assay

Article DOI: 10.3791/59164-v • 06:11 min • February 27th, 2019
February 27th, 2019 •
1, 2, 1, 2
1Department of Enteric Infections, Bacterial Diseases Branch, Walter Reed Army Institute of Research, 2University of Alabama at Birmingham

Chapters

Summary
Automatic Translation

Here we present a protocol to measure Shigellacidal activity of antibodies in serum. Serum is mixed with bacteria and exogenous complement, incubated, and the reaction mixture is plated on agar plates. Viable bacteria form colonies which are counted, using an automated colony enumerator, and used to determine the bactericidal titer.

Tags

High-throughput Shigella-specific Bactericidal Assay Antibodies Immunization Infection Immune Responses Bactericidal Activity High Throughput Analysis Assay Plate Assay Buffer Test Samples Serial Dilutions Multi-channel Pipette
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter