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Isolando il maligno e le cellule di B Non-maligna da lck
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Isolando il maligno e le cellule di B Non-maligna da lck:eGFP Zebrafish

Article DOI: 10.3791/59191-v • 08:32 min • February 22nd, 2019
February 22nd, 2019 •
1, *1, *1, 1, 1
1Section of Pediatric Hematology-Oncology, Department of Pediatrics, University of Oklahoma Health Sciences Center
* These authors contributed equally

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Zebrafish transgenici lck:eGFP esprimono GFP altamente nei linfociti T e sono stati usati per studiare lo sviluppo delle cellule T e la leucemia linfoblastica acuta. Questa linea può essere utilizzato per Studio B cellule, che esprimono lck a livelli inferiori. Questo protocollo descrive la purificazione delle cellule di B maligne e benigne da lck:eGFP zebrafish.

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Medicina problema 144 Zebrafish d. rerio leucemia la leucemia linfoblastica acuta linfociti citometria a flusso
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