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Aislar maligno y no maligno de células B lck:eGFP pez cebra

Article DOI: 10.3791/59191-v • 08:32 min • February 22nd, 2019
February 22nd, 2019 •
1, *1, *1, 1, 1
1Section of Pediatric Hematology-Oncology, Department of Pediatrics, University of Oklahoma Health Sciences Center
* These authors contributed equally

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Pez cebra transgénico lck:eGFP expresan GFP altamente en los linfocitos T y se han utilizado para estudiar el desarrollo de las células T y leucemia linfoblástica aguda. Esta línea puede utilizarse para células de estudio B, que expresan lck en niveles inferiores. Este protocolo describe la purificación de las células B malignas y no malignas del pez cebra lck:eGFP .

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Medicina edición 144 pez cebra D. rerio leucemia leucemia linfoblástica aguda los linfocitos citometría de flujo
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