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Journal / Medicine / Isolera elakartat och icke-elakartat B-celler…
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Isolera elakartat och icke-elakartat B-celler från lck
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Isolera elakartat och icke-elakartat B-celler från lck:eGFP Zebrafiskar

Article DOI: 10.3791/59191-v • 08:32 min • February 22nd, 2019
February 22nd, 2019 •
1, *1, *1, 1, 1
1Section of Pediatric Hematology-Oncology, Department of Pediatrics, University of Oklahoma Health Sciences Center
* These authors contributed equally

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Transgena lck:eGFP Sebrafisken uttrycker GFP högt i T-lymfocyter, och har använts för att studera T cell utveckling och akut lymfatisk leukemi. Denna linje kan användas till studie B-celler, som uttrycker lck på lägre nivåer. Det här protokollet beskriver rening av maligna och icke-maligna B-celler från lck:eGFP Zebrafiskar.

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Medicin fråga 144 Zebrafiskar D. rerio leukemi akut lymfatisk leukemi lymfocyter flödescytometri
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