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Genetics

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JoVE Journal Genetics
Verbesserte Hefe One-Hybrid Screens, Transkriptionsfaktor Bindung an menschliche DNA-Sequenzen zu identifizieren
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Verbesserte Hefe One-Hybrid Screens, Transkriptionsfaktor Bindung an menschliche DNA-Sequenzen zu identifizieren

Article DOI: 10.3791/59192-v • 11:25 min • February 11th, 2019
February 11th, 2019 •
*1, *1, *1, 1,2
1Department of Biology, Boston University, 2Bioinformatics Program, Boston University
* These authors contributed equally

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Hier präsentieren wir Ihnen eine erweiterte Hefe One-Hybrid screening Protokoll um den Transkriptionsfaktoren (TFs) zu identifizieren, die an einer menschlichen DNA-Region of Interest binden können. Diese Methode nutzt eine Hochdurchsatz-Screening-Pipeline, die die Bindung von Verhören kann > 1.000 TFs in einem einzigen Experiment.

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Genetik Ausgabe 144 Hefe One-Hybrid Y1H Transkriptionsfaktor Mensch gen Verordnung DNA eY1H
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