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JoVE Journal Cancer Research
Intramucosal podning af pladecellekræft celler i mus til Tumor immun profilering og behandling svar vurdering
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Intramucosal podning af pladecellekræft celler i mus til Tumor immun profilering og behandling svar vurdering

Article DOI: 10.3791/59195-v • 07:29 min • April 22nd, 2019
April 22nd, 2019 •
1, 1, 1, 1, 1,2,3, 1
1Department of Radiation Oncology, University of Colorado Denver - Anschutz Medical Campus, 2Department of Anesthesiology, University of Colorado Denver - Anschutz Medical Campus, 3Division of Radiology, University of Colorado Denver - Anschutz Medical Campus

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Her præsenterer vi en reproducerbar metode til at udvikle en orthotopic murine model af hoved og hals planocellulært karcinom. Tumorer påvise klinisk relevante histopatologiske funktioner af sygdom, herunder nekrose, dårlig differentiering, nodal metastaser og immun infiltration. Tumor-bærende mus udvikler klinisk relevante symptomer herunder dysfagi, kæben forskydning og vægttab.

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Kræftforskning spørgsmålet 146 hoved og hals kræft orthotopic model tumor mikromiljø immun unddragelse flowcytometri masse flowcytometri
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