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Journal / Biochemistry / プロテオーム的分類の単一分子レベルでの均一性の定量化…
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Biochemistry

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JoVE Journal Biochemistry
プロテオーム的分類の単一分子レベルでの均一性の定量化
Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
Journal (Biochemistry)
Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
Journal (Biochemistry)
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
 
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プロテオーム的分類の単一分子レベルでの均一性の定量化

Article DOI: 10.3791/59199-v • 08:29 min • April 19th, 2019
April 19th, 2019 •
1,2, 2, 1,3
1Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research, RIKEN, 2Laboratoire de Biomatériaux et Bioimagerie, INSERM, 3PRESTO, Japan Science and Technology Agency

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ここでは、単一分子レベルの複雑な蛋白質のサンプルのそれぞれのタンパク質種のラベリングの均質性を評価するためのプロトコルを提案する.

Tags

生化学、問題 146、1 分子イメージング、プロテオミクス、蛍光標識、ゲル電気泳動、均質性、細胞ライセートをラベリング
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