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Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
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Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Article DOI: 10.3791/59212-v • 05:44 min • March 1st, 2019
March 1st, 2019 •
1,2, 1,2, 1,2, 1,2, 1,2
1Department of Growth and Reproduction, Copenhagen University Hospital, 2International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), University of Copenhagen

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Summary
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Here, two medium-throughput assays for assessment of effects on Ca2+-signaling and acrosome reaction in human sperm are described. These assays can be used to quickly and easily screen large amounts of compounds for effects on Ca2+-signaling and acrosome reaction in human sperm.

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Medium-throughput Screening Assays Assessment Effects Ca2+-signaling Acrosome Reaction Human Sperm Compounds Function Calcium Signaling Acrosome Reaction Visual Demonstrations Experiments Labs Semen Sample Tube Rack Human Tubal Fluid (HTF) Incubation Motile Sperm Cells Counting Sperm Cells Dilution Factor S100 Buffer SP1 Cassette
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