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Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations
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Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

Article DOI: 10.3791/59214-v • 05:22 min • March 21st, 2019
March 21st, 2019 •
1, 2, 3
1NanoSquare Research Institute, Osaka Prefecture University, 2Department of Bioscience and Informatics, Osaka Prefecture University, 3Department of Biological Functions Engineering, Kyushu Institute of Technology

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Summary
Automatic Translation

We present a procedure for controlling the initial cell cluster shape in a 3D extracellular matrix to obtain a repeatable pattern formation. A cubic device containing two different hydrogels is employed to achieve multi-directional imaging for tissue pattern formation.

Tags

3D Cell Cluster Control Hybrid Gel Cube Device Repeatable Pattern Formations 3D Culture Experiment Initial Culture Condition Polycarbonate Cubic Frame Agarose Extracellular Matrix Micro Mold Carbon Dioxide Incubator
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