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JoVE Journal
Biochemistry

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At studere RNA Interactors af Protein Kinase RNA-aktiveret under pattedyr cellecyklus
 
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At studere RNA Interactors af Protein Kinase RNA-aktiveret under pattedyr cellecyklus

Article DOI: 10.3791/59215-v 10:05 min March 5th, 2019
March 5th, 2019

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Summary

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Click here for the English version.

Vi præsenterer eksperimentelle metoder til at studere RNA-interactors af dobbelt-strenget RNA bindende protein kinase RNA-aktiveret (PKR) i løbet af pattedyr celle cyklus ved hjælp af HeLa celler. Denne metode udnytter formaldehyd bitmapgenkendelse RNA-PKR komplekser og immunoprecipitation til at berige PKR-bundet RNA'er. Disse RNA'er kan analyseres yderligere gennem høj overførselshastighed sekvensering eller qRT-PCR.

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Biokemi sag 145 dobbelt-strenget RNA mitokondrier RNA RNA bindende protein RNA-RBP interaktion protein kinase RNA-aktiveret formaldehyd crosslinking celle cyklus strand-specifikke qRT-PCR
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