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Journal / Biochemistry / Studere RNA interaktører av Protein Kinase…
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Biochemistry

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JoVE Journal Biochemistry
Studere RNA interaktører av Protein Kinase RNA-aktivert under pattedyr cellen syklus
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Studere RNA interaktører av Protein Kinase RNA-aktivert under pattedyr cellen syklus

Article DOI: 10.3791/59215-v • 10:05 min • March 5th, 2019
March 5th, 2019 •
1, 1, 1
1Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

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Vi presenterer eksperimentelle metoder for studere RNA-interaktører av double-strandet RNA bindende protein kinase RNA-aktivert (PKR) under pattedyr cellen syklus bruker HeLa celler. Denne metoden bruker formaldehyd krysskobling RNA-PKR komplekser og immunoprecipitation å berike PKR-bundet RNAs. Disse RNAs kan analyseres videre gjennom høy gjennomstrømming sekvensering eller qRT PCR.

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Biokjemi problemet 145 Double-strandet RNA mitokondrie RNA RNA bindende protein RNA-RBP samhandling protein kinase RNA-aktivert formaldehyd crosslinking celle syklus strand-spesifikke qRT PCR
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