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Journal / Immunology and Infection / Generasjon, forsterkning og titrering rekombinant …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Generasjon, forsterkning og titrering rekombinant respiratorisk Syncytial virus
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Generasjon, forsterkning og titrering rekombinant respiratorisk Syncytial virus

Article DOI: 10.3791/59218-v • 11:48 min • April 4th, 2019
April 4th, 2019 •
1, 1, 1, 1, 1, 2, 1,3, 1,3
1UMR 1173 Institut national de la santé et de la recherche médicale (INSERM), Université de Versailles St. Quentin, 2UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires, 3Assistance Publique–Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie

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Vi beskriver en metode for å generere og forsterke genetisk endret respiratorisk syncytial virus (RSVs) og en optimalisert plakk analysen for RSVs. Vi illustrerer denne protokollen ved å opprette to rekombinant virus som henholdsvis tillate kvantifisering av RSV replikering og live analyse av RSV inkludering organer og inkludering organer-assosiert granulater dynamikk.

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Immunologi og infeksjon problemet 146 omvendt genetikk respiratorisk syncytial virus infeksjon rekombinant virus forsterkning titrering tag fluorescens kvantifisering video mikroskopi
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