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Journal / Neuroscience / En menneskelige blod-hjerne Interface Model til…
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Neuroscience

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JoVE Journal Neuroscience
En menneskelige blod-hjerne Interface Model til at studere barriere overfarter af patogener eller lægemidler og deres interaktioner med hjernen
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En menneskelige blod-hjerne Interface Model til at studere barriere overfarter af patogener eller lægemidler og deres interaktioner med hjernen

Article DOI: 10.3791/59220-v • 07:52 min • April 9th, 2019
April 9th, 2019 •
*1, *1, 1, 2, 2, 3, 1
1Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale, 2Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot, 3Institut Pasteur, PFIA, DTPS
* These authors contributed equally

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Her præsenterer vi en protokol om fastsættelse af en i cellulo BBB (blod-hjerne barrieren)-Minibrain polyester porøse membran kultur indsætte system for at evaluere transport af biomolekyler eller infektiøse agenser på tværs af en menneskelig BBB og deres fysiologisk indvirkning på de nærliggende hjerneceller.

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Neurovidenskab spørgsmålet 146 BBB-Minibrain i cellulo model menneskelige endotelceller hCMEC/D3 Ntera/Cl2D.1 menneskelige neuron menneskelige astrocyte menneskelige microglial celler CHME/Cl5
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