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JoVE Journal Bioengineering
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3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Article DOI: 10.3791/59226-v • 05:57 min • May 24th, 2019
May 24th, 2019 •
1, 2, 1, 2,3, 1
1Department of Biomedical Engineering and Yale Systems Biology Institute, Yale University, 2Department of Biomedical Engineering, Johns Hopkins University, 3Center for Cell Dynamics and Department of Cell Biology, Johns Hopkins University

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Summary
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We describe a method to construct devices for 3D culture and experimentation with cells and multicellular organoids. This device allows analysis of cellular responses to soluble signals in 3D microenvironments with defined chemoattractant gradients. Organoids are better than single cells at detection of weak noisy inputs.

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3D Analysis Multi-cellular Responses Chemoattractant Gradients 2D In Vitro Cultures 3D Tissue-like Environments Fabrication Process Photolithography Techniques 3D PDMS Device Mesofluidic Device Preparation Computer-aided Design Software Mold Design Polydimethylsiloxane (PDMS) Device Stereo Lithography Equipment Thermoresistant Resin PDMS Monomer Solution Curing Agent Vacuum Desiccator Degassing Adhesive Tape PDMS Solution Curing PDMS Boundary Cutting Coverslip Fitting
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