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Journal / Genetics / Overekspression længe Noncoding RNA'er ved hjælp…
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Genetics

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JoVE Journal Genetics
Overekspression længe Noncoding RNA'er ved hjælp af gen-aktivering CRISPR
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Overekspression længe Noncoding RNA'er ved hjælp af gen-aktivering CRISPR

Article DOI: 10.3791/59233-v • 13:04 min • March 1st, 2019
March 1st, 2019 •
1, 1, 2, 1, 3, 3, 1, 1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

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Traditionelle cDNA-baserede overekspression teknikker har en begrænset anvendelighed for overekspression af lange noncoding RNA'er på grund af deres flere splice formularer med potentielle funktionalitet. Denne revision rapporterer en protokol, ved hjælp af CRISPR teknologi til overexpress flere splice varianter af en længe noncoding RNA.

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Genetik sag 145 lncRNA CRISPR IFNG-AS1 overekspression lentiviral real-time PCR
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