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Journal / Genetics / Überexprimierenden lange forensisches RNAs mit…
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Genetics

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JoVE Journal Genetics
Überexprimierenden lange forensisches RNAs mit Gen-Aktivierung CRISPR
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Journal (Genetics)
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Überexprimierenden lange forensisches RNAs mit Gen-Aktivierung CRISPR

Article DOI: 10.3791/59233-v • 13:04 min • March 1st, 2019
March 1st, 2019 •
1, 1, 2, 1, 3, 3, 1, 1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

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Traditionelle cDNA-basierte Überexpression Techniken haben eine begrenzte Anwendbarkeit für die Überexpression von lange forensisches RNAs aufgrund ihrer mehrere Spleiß-Formulare mit potenziellen Funktionalität. Dieser Beitrag berichtet ein Protokoll mit CRISPR-Technologie mehrere Splice-Varianten eine lange nichtcodierender RNA overexpress.

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Genetik Ausgabe 145 LncRNA CRISPR IFNG-AS1 Überexpression Lentivirale Real-Time PCR
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