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Journal / Genetics / लंबे noncoding rnas का उपयोग करके overexpressing…
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JoVE Journal Genetics
लंबे noncoding rnas का उपयोग करके overexpressing जीन सक्रिय crispr
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लंबे noncoding rnas का उपयोग करके overexpressing जीन सक्रिय crispr

Article DOI: 10.3791/59233-v • 13:04 min • March 1st, 2019
March 1st, 2019 •
1, 1, 2, 1, 3, 3, 1, 1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

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पारंपरिक cdna आधारित overexpression तकनीकों लंबे noncoding की overexpression के लिए एक सीमित प्रयोज्यता है rnas संभावित कार्यक्षमता के साथ अपने कई ब्याह रूपों के कारण. यह समीक्षा एक लंबे noncoding आरएनए के कई ब्याह वेरिएंट overexpress करने के लिए crispr प्रौद्योगिकी का उपयोग कर एक प्रोटोकॉल की रिपोर्ट ।

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आनुवंशिकी मुद्दा १४५ lncrna crispr ifng-AS1 ओवरएक्सप्रेशन लेंवायरल रीयल-टाइम पीसीआर
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