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Journal / Genetics / In-vivo Anwendung der REMOTE-Steuerung für die…
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Genetics

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JoVE Journal Genetics
In-vivo Anwendung der REMOTE-Steuerung für die Manipulation von endogenen Genexpression
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In-vivo Anwendung der REMOTE-Steuerung für die Manipulation von endogenen Genexpression

Article DOI: 10.3791/59235-v • 08:54 min • March 29th, 2019
March 29th, 2019 •
1, 2, 1, 1, 1, 1
1Center for Epigenetics, Van Andel Research Institute, 2Amgen

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Dieses Protokoll beschreibt die Schritte erforderlich, um ein Modellsystem, in dem die Transkription des endogenen Gens von Interesse bedingt gesteuert werden kann, in lebenden Tieren oder Zellen mit verstärkten Lac Repressor und/oder Tet Aktivator Systeme generieren.

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Genetik Ausgabe 145 Transkription Ausdruck Reversible Lac Repressor Tet Aktivator Dnmt1 Repression Genregulation induzierbaren
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