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Journal / Genetics / Lentiviral Vector plattform för effektiv…
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JoVE Journal Genetics
Lentiviral Vector plattform för effektiv distribution av epigenomet-redigeringsverktyg i mänskliga inducerade pluripotenta stamceller-derived sjukdomsmodeller
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Lentiviral Vector plattform för effektiv distribution av epigenomet-redigeringsverktyg i mänskliga inducerade pluripotenta stamceller-derived sjukdomsmodeller

Article DOI: 10.3791/59241-v • 13:47 min • March 29th, 2019
March 29th, 2019 •
1,2, 3, 3, 1, 1,2, 4, 4, 1,2, 3
1Department of Neurology, Duke University Medical Center, 2Center for Genomic and Computational Biology, Duke University Medical Center, 3Viral Vector Core, Duke University Medical Center, 4Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Duke University Medical Center

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Riktade DNA epigenomet redigering representerar en kraftfull terapeutisk metod. Det här protokollet beskriver produktion, rening och koncentration av allt-i-ett lentiviral vektorer härbärgerat den CRISPR-dCas9-DNMT3A transgenens för epigenomet redigeringsprogram i mänskliga inducerade pluripotenta stamceller (hiPSC)-härledda nervceller.

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Genetik fråga 145 lentiviral vektorer DNMT3A CRISPR-dCas9 SNCA hiPSCs metylering pyrosekvensering epigenomet-redigering Parkinsons sjukdom
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