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Journal / Genetics / Plataforma de vectores lentivirales para la…
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Genetics

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JoVE Journal Genetics
Plataforma de vectores lentivirales para la entrega eficiente de las herramientas de edición de epigenoma en humanos inducida por modelos de enfermedad derivados de células madre pluripotentes
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Plataforma de vectores lentivirales para la entrega eficiente de las herramientas de edición de epigenoma en humanos inducida por modelos de enfermedad derivados de células madre pluripotentes

Article DOI: 10.3791/59241-v • 13:47 min • March 29th, 2019
March 29th, 2019 •
1,2, 3, 3, 1, 1,2, 4, 4, 1,2, 3
1Department of Neurology, Duke University Medical Center, 2Center for Genomic and Computational Biology, Duke University Medical Center, 3Viral Vector Core, Duke University Medical Center, 4Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Duke University Medical Center

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A ADN epigenoma edición representa un potente enfoque terapéutico. Este protocolo describe la producción, purificación y concentración de vectores lentivirales all-in-one que el transgén CRISPR-dCas9-DNMT3A para aplicaciones de edición de epigenoma en células pluripotentes inducidas humanas (hiPSC)-derivado de las neuronas.

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Genética número 145 vectores lentivirales DNMT3A CRISPR-dCas9 SNCA hiPSCs metilación pirosecuenciación edición de epigenoma la enfermedad de Parkinson
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