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Journal / Neuroscience / Dissectie van lokale Ca2 + signalen in gekweekte…
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Neuroscience

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JoVE Journal Neuroscience
Dissectie van lokale Ca2 + signalen in gekweekte cellen door membraan-gerichte Ca2 + indicatoren
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Dissectie van lokale Ca2 + signalen in gekweekte cellen door membraan-gerichte Ca2 + indicatoren

Article DOI: 10.3791/59246-v • 11:33 min • March 22nd, 2019
March 22nd, 2019 •
1,2, 2, 2,3, 2
1Japan Science and Technology Agency, PRESTO, 2Laboratory for Developmental Neurobiology, RIKEN Center for Brain Science, 3École Normale Supèrieure, Institut de Biologie de l'ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University

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Hier presenteren we een protocol voor Ca2 + imaging in zenuwcellen en gliacellen, waarmee de dissectie van Ca2 + signalen bij subcellular resolutie. Dit proces is van toepassing op alle celtypes waarmee de expressie van genetisch gecodeerde Ca2 + indicatoren.

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Neurowetenschappen kwestie 145 biologische Disciplines van de computerwetenschap biologie celbiologie neurowetenschappen neurobiologie Disciplines en beroepen natuurwetenschappen Disciplines Ca2 + beeldvorming lokale Ca2 + GCaMP6f RCaMP2 Ca2 + toestroom CA2 + release plasmamembraan endoplasmatisch reticulum cellijn neuron Astrocyt gedissocieerde cultuur
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